Parvalbumin, a cross-reactive fish allergen, contains IgE-binding epitopes sensitive to periodate treatment and Ca2+ depletion - 09/09/11
Abstract |
Background: Type I allergy to fish is a severe health problem in countries in which a large percentage of the population derive income from fishing. Objective: The aim of the study was to characterize cross-reactive IgE-binding components in six different fish species (cod, tuna, salmon, perch, carp, and eel). The effect of reducing extraction conditions, periodate treatment, and depletion of Ca2+ on binding of IgE to the allergens was investigated. Methods: Extracts were prepared under nonreducing and reducing conditions. IgE-binding components were characterized by IgE immunoblotting, and cross-reactive epitopes were studied by IgE-immunoblot inhibition experiments. To reveal calcium-sensitive or carbohydrate-containing epitopes, nitrocellulose-blotted extracts were exposed to ethylene glycol bis(β-aminoethyl ether)-N,N,N ́,N ́-tetraacetic acid (EGTA) and periodate. Results: Sera from all patients allergic to fish (n = 30) displayed IgE reactivity to parvalbumin, a 12 kd protein present in fish extracts from six different species. Reducing extraction conditions had no effect on IgE binding to parvalbumins, whereas periodate treatment and depletion of protein-bound calcium led to a substantial reduction of IgE binding. Parvalbumins from six different species contained cross-reactive IgE epitopes. Conclusion: Parvalbumin represents a cross-reactive fish allergen. It contains IgE epitopes that are sensitive to periodate treatment and Ca2+-depletion.
Le texte complet de cet article est disponible en PDF.Keywords : Fish allergens, immunoblot, cross-reactivity, parvalbumin, Ca2+-binding protein, IgE epitopes
Abbreviations : EGTA, SDS-PAGE
Plan
 | From the aInstitute of Medical and Chemical Laboratory Diagnostics, AKH, University of Vienna; bPharmacia-Upjohn Diagnostics, Uppsala; cDepartment of Dermatology, Georg-August University Göttingen; and dthe Institute of General and Experimental Pathology, AKH, University of Vienna. |
| Supported by a grant of the Bürgmeisterfonds Vienna and by grant F0506 of the Austrian Science Foundation. |
 | Reprint requests: Rudolf Valenta, MD, Institute of General and Experimental Pathology, AKH, University of Vienna, Währinger Gürtel 18-20, A-1090 Vienna, Austria. |
| 1/1/85504 |
Vol 101 - N° 1
P. 67-74 - janvier 1998 Retour au numéro
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