Comparative studies of functional and binding assays for IgG anti-FcϵRI⍺ (⍺-subunit) in chronic urticaria - 09/09/11
Abstract |
Background: Recent data suggest that a subpopulation of patients with chronic urticaria have an autoimmune disorder that is caused by the presence of antibodies to the IgE receptor. The actual incidence of these antibodies is uncertain. Objective: We sought to assess the incidence of autoimmunity to the IgE receptor in patients with chronic urticaria and to compare functional and binding assays. Methods: We isolated skin mast cells and studied a large number of patient sera (68) for their ability to activate these cells and isolated basophils. We then compared the results with those obtained by immunoblotting using cloned ⍺-subunit of the IgE receptor. Results: Sera from patients with chronic urticaria released significant histamine (>15% of basal) on incubation with basophils (48%) and mast cells (46%). By immunoblotting we obtained positive results in 64% of subjects tested and also identified a small subpopulation that is active on cells but does not bind FcϵRI⍺. Conclusion: Our data suggest that approximately 45% to 50% of patients with chronic urticaria have a cutaneous autoimmune disorder. Immunoblotting may provide a rapid screening method for anti-FcϵRI⍺ detection in such patients. (J Allergy Clin Immunol 1998;101:672-6.)
Le texte complet de cet article est disponible en PDF.Keywords : Urticaria, mast cells, histamine release, IgE receptor, autoimmunity
Plan
 | From athe Division of Pulmonary-Critical Care and Allergy-Immunology, Medical University of South Carolina, Charleston; and bthe Laboratory of Allergy and Immunology, Department of Pathology, Harvard Medical School, Boston. |
| Supported by grant #97/5083 from the Fondo de Investigación Sanitaria from the Health Department of Spain. |
 | Reprint requests: Allen P. Kaplan, MD, Department of Medicine, Division of Pulmonary Critical Care and Allergy and Clinical Immunology, Medical University of South Carolina, 171 Ashley Ave., Charleston, SC 29425. |
| 1/1/88662 |
Vol 101 - N° 5
P. 672-676 - mai 1998 Retour au numéro
Article précédent
- Determinant analysis of IgE and IgG4 antibodies and T cells specific for bovine ⍺s1-casein from the same patients allergic to cow's milk: Existence of ⍺s1-casein–specific B cells and T cells characteristic in cow's-milk allergy
- Haruyo Nakajima-Adachi, Satoshi Hachimura, Wataru Isea, Kiri Honma, Shinya Nishiwakia, Maiko Hirotaa, Naoki Shimojo, Toshiyuki Katsuki, Akio Ametani, Yoichi Kohno, Shuichi Kaminogawa
- IL-3, IL-5, granulocyte-macrophage colony- stimulating factor receptor ⍺-subunit, and common β-subunit expression by peripheral leukocytes and blood dendritic cells
- Takashi Yamada, Qiyu Sun, Kyriaki Zeibecoglou, Jatinder Bungre, Janet North, A.Barry Kay, Angel F. Lopez, Douglas S. Robinson
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Déjà abonné à cette revue ?