Herpes simplex virus type 2 detection by culture and polymerase chain reaction and relationship to genital symptoms and cervical antibody status during the third trimester of pregnancy - 10/09/11
Abstract |
OBJECTIVES: Our goal was to define the frequency of asymptomatic herpes simplex virus type 2 shedding by culture and polymerase chain reaction and to correlate our findings with cervical anti–herpes simplex virus type 2 immunoglobulin A production.
STUDY DESIGN: Women who were seropositive for herpes simplex virus type 2 collected daily genital tract samples during the third trimester for culture and deoxyribonucleic acid quantitation by polymerase chain reaction. Cervical secretions were collected weekly for anti–herpes simplex virus type 2 immunoglobulin A. Asymptomatic shedding by culture versus polymerase chain reaction and anti–herpes simplex virus type 2 immunoglobulin A detection with and without genital shedding were compared by means of McNemar's χ2 test.
RESULTS: Asymptomatic shedding was more frequent by polymerase chain reaction than by culture (13.8% vs 2.3%, p < 0.0001). When cervical anti–herpes simplex virus type 2 immunoglobulin A was present, patients were more likely to have negative results by polymerase chain reaction than positive results (66.7% vs 26.7%, p = 0.001). Anti–herpes simplex virus type 2 immunoglobulin A was detected beyond 37 weeks in only one subject.
CONCLUSIONS: Polymerase chain reaction was more sensitive than culture for detecting asymptomatic genital herpes simplex virus. The role of immunoglobulin A in clearing genital herpes simplex virus remains to be determined. (Am J Obstet Gynecol 1997;176:443-51.)
Le texte complet de cet article est disponible en PDF.Keywords : genital herpes simplex virus, polymerase chain reaction, immunoglobulin A
Plan
 | From the Departments of Obstetrics and Gynecologya and Laboratory Medicine,b University of Washington Medical Center. |
| Supported by National Institute of Allergy and Infectious Diseases training grant AI-01740-17 and Program Project grant AI-30731-04. |
 | Reprint requests: Kim A. Boggess MD, Department of Obstetrics and Gynecology, Duke University Medical Center, Box 3967, Durham, NC 27710. |
| 6/1/78619 |
Vol 176 - N° 2
P. 443-451 - février 1997 Retour au numéro
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