Cellular immunity is activated and a TH-2 response is associated with early wheezing in infants after bronchiolitis - 11/09/11
Abstract |
Objective: To determine whether abnormalities of cellular immunity are present and linked to early wheezing after bronchiolitis.
Methods: We prospectively studied 26 infants hospitalized for a first episode of bronchiolitis and without any prior immune, cardiac, or respiratory disease. Blood was obtained at the time of enrollment and 5 months later for the assessment of the total cellular and differential counts, CD4+ (helper) and CD8+ (suppressor/cytotoxic) lymphocytes, and the activation markers CD23 (low-affinity immunoglobulin E receptor) and CD25 (interleukin-2 [IL-2] receptor). The cytokines interferon gamma (T-helper [TH] type-1 cytokine) and IL-4 (TH-2) were measured in plasma and in vitro after stimulation with IL-2 or with the house-dust mite (Dermatophagoides farinae) antigen. A daily log of episodes of wheezing was kept by parents after discharge.
Results: We found an increase in blood eosinophils, an increased percentage of CD4+, CD25+, and CD23 + lymphocytes in subjects at 5 months compared with the time of bronchiolitis and with healthy subjects of the same age (p <0.05). Plasma IL-4 levels, although not different from those of healthy subjects, also increased significantly. Peripheral blood lymphocytes from infants who wheezed produced more IL-4 in vitro, 5 months after bronchiolitis, in response to D. farinae antigen. In babies who wheezed, a positive correlation was found between the total number of days that wheezing occurred and the blood eosinophil count. Babies who wheezed more often (>20 days) had more peripheral blood basophils and eosinophils, and peripheral blood lymphocytes obtained from these subjects at the time of bronchiolitis produced less interferon gamma on stimulation with IL-2.
Conclusions: Bronchiolitis is followed by activation of cellular immunity, and early wheezing in infants is associated with a TH-2 response. (J Pediatr 1997;130:584-93)
Le texte complet de cet article est disponible en PDF.Abbreviations : ELISA, IL, NS, RSV, TH
Plan
 | From the Sainte-Justine, L. C. Simard, and André Viallet Research Centers, the Pulmonary Units of Sainte Justine and Notre Dame Hospitals, the Respiratory Health Network of Centers of Excellence of Canada, the University of Montreal, and the Meakins Christie Laboratories, McGill University, Montreal, Quebec, Canada. |
| Supported in part by grants from the Quebec Thoracic Society, the Respiratory Health Network of Centers of Excellence of Canada and the Ste-Justine Research Institute. Dr. Renzi is recipient of a Scholar award from the Fonds de Recherche en Santé du Québec. Dr. Yang was partly funded by a grant from Boehringer Ingelheim to the University of Montreal. Dr. Pedneault is recipient of a Clin ical Scholar award from the Fonds de Recherche en Santé du Québec. |
 | Reprint requests: P. M. Renzi, MD, Meakins Christie Laboratories, 3626 St-Urbain Street, Montreal, PQ, Canada H2X-2P2. |
| 0022-3476/97/$5.00+0 9/21/78302 |
Vol 130 - N° 4
P. 584-593 - avril 1997 Retour au numéro
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