Elevated IgG titer against the C region of streptococcal M protein and its immunodeterminants in patients with poststreptococcal acute glomerulonephritis - 11/09/11
From the Department of Pediatrics, Kouchi Red-Cross Hospital, Kouchi; the Department of Pediatrics, Kurume University School of Medicine, Kurume; and the Department of Genetics, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan.
Abstract |
Objective: To determine the immune responses to the streptococcal M protein in patients with poststreptococcal acute glomerulonephritis (PSAGN).
Study design: The gene coding type 12 M protein of group A streptococcus (M12), a known PSAGN-associated serotype, was cloned and expressed in Escherichia coli to investigate the specific immune responses to the M12 protein in patients with PSAGN. Recombinant M proteins for the variable N-terminal half (AB region) and conserved C-terminal half (C region) were produced separately. IgG titers against each region were measured by enzyme-linked immunosorbent assay in patients with PSAGN ( n = 51), uncomplicated streptococcal pharyngitis ( n = 26), chronic glomerulonephritis ( n = 10), and in healthy control subjects ( n = 51). Immunodominant domains within the M protein in PSAGN were further investigated by use of overlapping synthetic peptides.
Results: IgG titers against the C region, but not the AB region, were markedly higher in the PSAGN group than in other groups ( p < 0.01), and these titers were maintained for at least several months after antistreptolysin O or antistreptokinase levels had returned to normal. Studies with overlapping synthetic peptides demonstrated that increased IgG reactivity was observed against the C repeat blocks.
Conclusion: IgG titers against the C region are significantly elevated in patients with PSAGN, and it may be a diagnostic marker for PSAGN. (J Pediatr 1997;131:293-9)
Le texte complet de cet article est disponible en PDF.Abbreviations : ASK, ASO, PCR, PSAGN
Plan
 | Reprint requests: Kazuhiro Mori, MD, Department of Pediatrics, Kouchi Red-Cross Hospital, 2-13-51, Shinhon-machi, Kouchi-city, 780, Japan. |
| 0022-3476/97/$5.00 + 0 9/21/79822 |
Vol 131 - N° 2
P. 293-299 - août 1997 Retour au numéro
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