Semen Parameters and Sperm DNA Fragmentation as Causes of Recurrent Pregnancy Loss - 07/10/11
, Meriem Mehdi, Hanène Landolsi, Soumaya Mougou, Hatem Elghezal, Ali Saad
Reprint requests: Sonia Brahem, M.D., Department of Cytogenetic and Reproductive Biology, Farhat Hached, University Teaching Hospital, 4000 Sousse, TunisiaRésumé |
Objectives |
To evaluate and compare standard sperm parameters, and sperm DNA fragmentation in seminal ejaculates from men whose partners had a history of recurrent pregnancy loss (RPL) and in a control group of men who had recently established their fertility.
Methods |
Semen samples from 31 patients with a history of recurrent pregnancy loss and 20 men with proven fertility were analyzed according to World Health Organization guidelines. Sperm DNA fragmentation was detected by the terminal deoxynucleotidyl transferase–mediated dUTP nick-end labeling assay.
Results |
When sperm quality of the control group was compared with that of the RPL group, a significant difference was observed in sperm motility, but not in other parameters. The mean number of sperm cells with fragmented DNA was significantly increased in the RPL group (32.22 ± 6.14%) compared with control donors (10.20 ± 2.1%).
Conclusions |
Our data indicate that sperm from men with a history of RPL have a higher incidence of DNA damage and poor motility than sperm from a control group, and this can explain in part the pregnancy loss in these patients.
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Vol 78 - N° 4
P. 792-796 - octobre 2011 Retour au numéro
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