Antioxidants modulate the antiproliferative effects of nitric oxide on vascular smooth muscle cells and adventitial fibroblasts by regulating oxidative stress - 19/10/11
Corresponding author. Tel.: +1-312-503-6701; fax: +1-312-503-1222Abstract |
Background |
S-nitrosothiols (SNO) release nitric oxide (NO) through interaction with ascorbic acid (AA). However, little is known about their combined effect in the vasculature. The aim of this study was to investigate the effect of AA on SNO-mediated NO release, proliferation, cell cycle progression, cell death, and oxidative stress in vascular cells.
Methods |
Vascular smooth muscle cells and adventitial fibroblasts harvested from the aortae of Sprague–Dawley rats were treated with AA, ± S-nitrosoglutathione (GSNO), or ± diethylenetriamine NONOate (DETA/NO). NO release, proliferation, cell cycle progression, cell death, and oxidative stress were determined by the Griess reaction, [3H]-thymidine incorporation, flow cytometry, trypan blue exclusion, and 5-(and-6)chloromethyl-2′,7′dichlorodihydrofluorescein staining, respectively.
Results |
AA increased NO release from GSNO 3-fold (P < .001). GSNO and DETA/NO significantly decreased proliferation, but AA abrogated this effect (P < .05). Mirroring the proliferation data, changes in cell cycle progression induced by GSNO and DETA/NO were reversed by the addition of AA. GSNO- and DETA/NO-mediated increases in oxidative stress were significantly decreased by the addition of AA (P < .001).
Conclusions |
Despite causing increased NO release from GSNO, AA reduced the antiproliferative and cell cycle effects of GSNO and DETA/NO through the modulation of oxidative stress.
Le texte complet de cet article est disponible en PDF.Keywords : Atherosclerosis, Peripheral arterial disease, Vascular smooth muscle cell proliferation, Ascorbic acid, Reactive oxygen species, Nitric oxide
Plan
| Supported by funding from the National Institutes of Health (K08HL084203, MRK), (T32 HL094293-01, EKG), the Society for Vascular Surgery Foundation (MRK) and Northwestern Memorial Foundation Collaborative Development Initiative, Center for Limb Preservation, Bluhm Cardiovascular Institute. |
Vol 202 - N° 5
P. 536-540 - novembre 2011 Retour au numéro
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