Protease-activated receptor 2–dependent fluid secretion from airway submucosal glands by house dust mite extract - 03/02/12
, ‡, Hyun Jae Lee, MSc b, c, e, ‡, Sang Cheol Kim, MD b, Kyubo Kim, MD a, Yoo Suk Kim, MD b, Chang-Hoon Kim, MD, PhD b, c, d, Jeung-Gweon Lee, MD, PhD b, Joo-Heon Yoon, MD, PhD b, c, d, e, ⁎ 
, Jae Young Choi, MD, PhD b, e, ⁎ Corresponding author: Jae Young Choi, MD, PhD, Department of Otorhinolaryngology, Yonsei University College of Medicine, 134 Shinchon-dong, Seodaemun-gu, Seoul, Korea 120-752.Joo-Heon Yoon, MD, PhD, Department of Otorhinolaryngology, Yonsei University College of Medicine, 134 Shinchon-dong, Seodaemun-gu, Seoul, Korea 120-752.Abstract |
Background |
The submucosal gland (SMG) is important in the control of airway surface fluid. Protease-activated receptor (PAR) 2 contributes to the pathophysiology of allergies in response to nonspecific allergens bearing proteases and anion secretion. House dust mites (HDMs) have abundant proteases that can activate PAR2, but little is known about the direct effect of HDM on SMG secretion.
Objective |
The aim of this study was to investigate the effect of HDMs on glandular secretion and its mechanism in allergic patients, patients with chronic rhinosinusitis (CRS), or both.
Methods |
Inferior nasal turbinates were harvested from 55 patients and classified into 4 groups (the control, allergic rhinitis [AR], CRS, and AR+CRS groups). A microscope attached to a digital camera was used to quantify mucus bubbles from individual SMGs while stimulated with HDM extract, PAR2-activating peptide, and carbachol. PAR2 expression in the SMG was determined by means of immunostaining with anti-PAR2 mAb.
Results |
HDM induced a significantly higher secretion rate and number of responding glands in the AR and AR+CRS groups than in the control group. Interestingly, patients in the CRS group, who had no HDM-specific IgE antibody, showed a higher response than the control group, and its response was suppressed by a PAR2-selective antagonist. The responses to PAR2-activating peptide were similar to those to HDM, and their secretion rates positively correlated with HDM responses. PAR2 was highly expressed in all 3 disease groups with immunostaining.
Conclusions |
HDM allergens can induce glandular secretion in patients with AR, CRS, or both, and PAR2 represents a possible mechanism for nonspecific hyperreactivity in inflammatory airway diseases.
Le texte complet de cet article est disponible en PDF.Key words : House dust mites, submucosal gland, allergy, chronic rhinosinusitis, protease-activated receptor, nasal
Abbreviations used : AR, CaCC, CFTR, CRS, ENMD-1068, HDM, KRB, PAR, PAR2-AP, SMG
Plan
| Supported by a Korea Research Foundation Grant funded by the Korean Government (MOEHRD, Basic Research Promotion Fund [KRF-2007-313-E00337 to J.Y.C.]) and the National Research Foundation of Korea by the Ministry of Education, Science, and Technology (Basic Science Research Program, 2011-0001168 to J.-H.Y.). |
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| Disclosure of potential conflict of interest: The authors declare that they have no relevant conflicts of interest. |
Vol 129 - N° 2
P. 529 - février 2012 Retour au numéro
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