A MicroRNA Guide for Clinicians and Basic Scientists: Background and Experimental Techniques - 25/02/12
, Fadi J. Charchar b, Ruby C.Y. Lin c, Julie R. McMullen a, ⁎ 
Corresponding author at: P.O. Box 6492, St Kilda Road Central, Melbourne, Victoria 8008, Australia. Tel.: +61 385321167; fax: +61 385321100.Corresponding author at: P.O. Box 6492, St Kilda Road Central, Melbourne, Victoria 8008, Australia. Tel.: +61 385321194; fax: +61 385321100.Résumé |
MicroRNAs (miRNAs) are short non-coding RNA molecules that are approximately 22 nucleotides in length. In the last 10years, miRNA research and discovery has advanced at a rapid rate. This review provides a brief overview of the discovery and biology of miRNAs, and summarises some of the experimental techniques used for isolation, detection, target prediction, and regulation of miRNAs. We also outline experimental workflows for investigators new to the field, and discuss the diagnostic and therapeutic application of miRNAs.
Le texte complet de cet article est disponible en PDF.Keywords : MicroRNAs, Biomarkers, Research tools, Experimental techniques, Therapeutics
Plan
Vol 21 - N° 3
P. 131-142 - mars 2012 Retour au numéro
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