Human amnion epithelial cells reduce ventilation-induced preterm lung injury in fetal sheep - 26/04/12
Reprints: Euan M. Wallace, MD, The Ritchie Centre, Monash Institute of Medical Research, Monash University, Clayton, Victoria 3168, AustraliaRésumé |
Objective |
The objective of the study was to explore whether human amnion epithelial cells (hAECs) can mitigate ventilation-induced lung injury.
Study Design |
An established in utero ovine model of ventilation-induced lung injury was used. At day 110 of gestation, singleton fetal lambs either had sham in utero ventilation (IUV) (n = 4), 12 hours of IUV alone (n = 4), or 12 hours of IUV and hAEC administration (n = 5). The primary outcome, structural lung injury, was assessed 1 week later.
Results |
Compared with sham controls, IUV alone was associated with significant lung injury: increased collagen (P = .03), elastin (P = .02), fibrosis (P = .02), and reduced secondary-septal crests (P = .009). This effect of IUV was significantly mitigated by the administration of hAECs: less collagen (P = .03), elastin (P = .04), fibrosis (P = .02), normalized secondary-septal crests (P = .02). The hAECs were immunolocalized within the fetal lung and had differentiated into type I and II alveolar cells.
Conclusion |
The hAECs mitigate ventilation-induced lung injury and differentiated into alveolar cells in vivo.
Le texte complet de cet article est disponible en PDF.Key words : amnion epithelial cells, bronchopulmonary dysplasia, fetal sheep, ventilation-induced lung injury
Plan
| The authors report no conflict of interest. |
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| This study was supported by project grant 606438 from the National Health and Medical Research Council of Australia, a Glynn White Research Scholarship (R.J.H.) from the RANZCOG Research Foundation, and the Victorian Government's Operational Infrastructure Support Program. |
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| Cite this article as: Hodges RJ, Jenkin G, Hooper SB, et al. Human amnion epithelial cells reduce ventilation-induced preterm lung injury in fetal sheep. Am J Obstet Gynecol 2012;206:448.e8-15. |
Vol 206 - N° 5
P. 448.e8-448.e15 - mai 2012 Retour au numéro
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