Identification of a Hyperpolarization-activated Cyclic Nucleotide-gated Channel and Its Subtypes in the Urinary Bladder of the Rat - 31/05/12
Reprint requests: Li Longkun, M.D., Ph.D., Institute of Urology, Southwest Hospital, Third Military Medical University, Chongqing 400038, ChinaRésumé |
Objective |
To investigate the distribution and effects of hyperpolarization-activated cyclic nucleotide-gated (HCN) channel and its isoforms in bladder, especially in bladder interstitial cells of Cajal (ICC).
Methods |
Four HCN isoforms were detected in bladder tissue from rats using reverse transcription–polymerase chain reaction and Western blotting. The HCN1 subtype was observed in bladder ICCs by double-labeled fluorescence. The effect of the HCN blocker, ZD7288, was investigated using the bladder smooth muscle strip test.
Results |
HCN1-4 isoforms were all identified in bladder ICCs using reverse transcription–polymerase chain reaction and Western blotting. Based on our semiquantitative analysis, HCN1 was found to be the most prominent isoform. The expression of HCN1 was confirmed in bladder ICCs by double-labeled fluorescence through colabeling of HCN1 and kit (CD117). ZD7288 significantly decreased the bladder excitation.
Conclusion |
All 4 HCN channel isoforms exist in the bladder, and they affect the bladder excitation, presumably via bladder ICCs.
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| Financial Disclosure: The authors declare that they have no relevant financial interests. |
Vol 79 - N° 6
P. 1411.e7-1411.e13 - juin 2012 Retour au numéro
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