Pepsinized cashew proteins are hypoallergenic and immunogenic and provide effective immunotherapy in mice with cashew allergy - 29/08/12
, Ian MacQueen, BS, Yifan Li, BS, Rishu Guo, MD, PhD, Xiao-Ping Zhong, MD, PhD, A. Wesley Burks, MD
Corresponding author: Mike Kulis, PhD, Duke University Medical Center, Medical Science Research Bldg 1, Rm 141, Durham, NC 27710.Abstract |
Background |
IgE-mediated allergic reactions to cashews and other nuts can trigger life-threatening anaphylaxis. Proactive therapies to decrease reaction severity do not exist.
Objectives |
We aimed to determine the efficacy of pepsin-digested cashew proteins used as immunotherapy in a murine model of cashew allergy.
Methods |
Mice were sensitized to cashew and then underwent challenges with digested or native cashew allergens to assess the allergenicity of the protein preparations. Using native or pepsinized cashew proteins, mice underwent oral or intraperitoneal sensitization protocols to determine the immunogenic properties of the protein preparations. Finally, cashew-sensitized mice underwent an immunotherapy protocol with native or pepsinized cashew proteins and subsequent provocation challenges.
Results |
Pepsinized cashew proteins elicited weaker allergic reactions than native cashew proteins but importantly retained the ability to stimulate cellular proliferation and cytokine production. Mice sensitized with pepsinized proteins reacted on challenge with native allergens, demonstrating that pepsinized allergens retain immunogenicity in vivo. Immunotherapy with pepsinized cashew allergens significantly decreased allergic symptoms and body temperature decrease relative to placebo after challenge with native and pepsinized proteins. Immunologic changes were comparable after immunotherapy with native or pepsinized allergens: TH2-type cytokine secretion from splenocytes was decreased, whereas specific IgG1 and IgG2a levels were increased.
Conclusions |
Pepsinized cashew proteins are effective in treating cashew allergy in mice and appear to work through the same mechanisms as native protein immunotherapy.
Le texte complet de cet article est disponible en PDF.Key words : Food allergy, tree nut allergy, cashew, immunotherapy, pepsin, murine model
Abbreviations used : nCSH, pCSH
Plan
| Supported by an NRSA F32 Fellowship to M.K. (1F32AI084332-01). |
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| Disclosure of potential conflict of interest: M. Kulis has received research support from the National Institutes of Health (NIH)/National Institute of Allergy and Infectious Diseases (NIAID) and is employed by Duke University. R. Guo is employed by Duke University. X.-P. Zhong has received research support from the NIH, the American Cancer Society, and the Food Allergy & Anaphylaxis Network (FAAN). A. W. Burks has received research support from the NIAID/NIH, the FAAN, the Food Allergy Initiative, the NIH, the National Peanut Board, Scientific Hospital Supplies, and the Wallace Research Foundation; has received travel support from the NIAID/NIH, the American Academy of Allergy, Asthma & Immunology (AAAAI), the American College of Allergy, Asthma & Immunology, and the European Academy of Allergy and Clinical Immunology; is on the board for the AAAAI, the FAAN, the US Food and Drug Administration, the Journal of Allergy and Clinical Immunology, and the NIH HAI; has received consultancy fees from Dannon Co Probiotics, ExploraMed Development, Intelliject, McNeil Nutritionals, Merck & Co, Novartis, Nutricia, Pfizer, Portola Pharmaceuticals, and Schering-Plough; is employed by the Duke University Medical Center and UNC North Carolina Children’s Hospital; receives royalties from UpToDate; has received payment for educational presentations development from Current Views; and has stock/stock options in Allertein and MastCell. The rest of the authors declare that they have no relevant conflicts of interest. |
Vol 130 - N° 3
P. 716-723 - septembre 2012 Retour au numéro
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