Validation and comparison of three adenosine triphosphate luminometers for monitoring hospital surface sanitization: A Rosetta Stone for adenosine triphosphate testing - 28/09/12
, R. Allen Giles, BS b
Address correspondence to Carmen V. Sciortino, PhD, Chief of Microbiology and Associate Director of Molecular Pathology, Robley Rex Veterans Administration Medical Center, 800 Zorn Avenue, Louisville, KY 40206.Abstract |
Background |
The application of adenosine triphosphate (ATP) luminometry for monitoring sanitized hospital surfaces is an evolving infection control practice. The goal of this study is to validate and compare 3 commercially available ATP luminometry, swab, rapid-test systems for use in hospital surface sanitization testing. Regulatory agencies may consider this a point-of-care laboratory test; it is therefore imperative that validation criteria are established.
Methods |
The ability of instruments to measure long-term stability of ATP dried on surfaces was determined. Dilutions of 3 species of microorganisms and a blood sample were dried onto a surface and tested. The performance characteristics of instruments were compared side-by-side for their ability to recover microorganism-derived ATP from surfaces.
Results |
Timed studies showed that surface biologic-ATP remained detectable for 10 days. Instrument clinical sensitivity, precision, detection range, limit of detection, and linearity were determined. Swab recovery of microorganisms from surfaces varied by instrument and organism. All 3 systems detected microorganisms in the presence of disinfectants.
Conclusion |
All instruments were validated, but only 1 was verified, because of variations in performance for each system. These studies indicate that careful consideration of the technologic application and instrument performance are important criteria for the selection of an ATP monitoring system.
Le texte complet de cet article est disponible en PDF.Key Words : Linearity, Precision, Limit of detection
Plan
| This work was performed at the Robley Rex Department of Veterans Healthcare Administration, Louisville, KY, with the support of Infection Control, the VA VISN C difficile project, Environmental Management Services, the Employee’s Workers Union. |
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| Charm Sciences, Inc, and 3M Corporation provided loaner instruments and swabs at no charge in support of this study. Charm Sciences, Inc, 3M Corporation, and manufacturers of the Hygiena System Sure II instrument may either gain or lose financially from the results of this study. |
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| Conflicts of interest: C.V.S. is an employee of the Robley Rex Veterans Affairs Medical Center and performed these studies as a part of his official duties. He received no financial gain from his employer or from any outside source for the work performed. R.A.G. is an official volunteer at the Robley Rex Veterans Affairs Medical Center. He received no financial compensation for his work. |
Vol 40 - N° 8
P. e233-e239 - octobre 2012 Retour au numéro
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