Obesity and Hypercholesterolemia are Associated with NOX2 Generated Oxidative Stress and Arterial Dysfunction - 20/11/12
, Francesco Martino, MD 2, Roberto Carnevale, PhD 1, Pasquale Pignatelli, MD 1, Elisa Catasca, MD 1, Ludovica Perri, MD 1, Cinzia M. Calabrese, MD 1, Maria Michela Palumbo, MD 1, Francesco Baratta, MD 1, Maria Del Ben, MD 1, Francesco Angelico, MD 1, Francesco Violi, MD 1
Reprint requests: Lorenzo Loffredo, MD, I Clinica Medica, Sapienza University, Viale del Policlinico 155, 00161, Rome, Italy.Abstract |
Objective |
To analyze the interplay among oxidative stress, NOX2, the catalytic core of nicotinamide-adenine dinucleotide phosphate oxidase, and endothelial dysfunction in children with obesity and/or hypercholesterolemia.
Study design |
We performed a cross-sectional study comparing flow-mediated arterial dilation (FMD), oxidized low-density lipoprotein, and urinary excretion of isoprostanes (8-iso-PGF2⍺), as markers of oxidative stress, and NOX2 activity, as assessed by blood levels of soluble NOX2-dp (sNOX2-dp), in a population of 100 children, matched for age and sex, including 40 healthy subjects (HS), 20 children with hypercholesterolemia (HC), 20 obese children (OC), and 20 children with coexistence of hypercholesterolemia and obesity (HOC).
Results |
HOC had higher sNOX2-dp and oxidized low-density lipoprotein levels compared with HS, HC, and OC. HC, OC, and HOC had lower FMD values compared with HS. Urinary 8-iso-PGF2⍺ excretion was higher in HOC compared with HS. FMD was inversely correlated with sNOX2-dp levels (r = −0.483; P < .001) and with the number of cardiovascular risk factors (r = −0.617; P < .001). Multiple linear regression analysis showed that the number of cardiovascular risk factors was the only independent predictive variable associated with FMD (β: −0.585; P < .001; R2 = 35%) and sNOX2-dp (β: 0.587; P < .001; R2 = 34%).
Conclusion |
The study suggests that NOX2-generating oxidative stress may have a pathogenic role in the functional changes of the arterial wall occurring in HOC.
Le texte complet de cet article est disponible en PDF.Keyword : BMI, 8-iso-PGF2⍺, FMD, HC, HOC, HS, IMT, LDL, NADPH, NO, OC, ox-LDL, sNOX2-dp
Plan
| Supported by a grant from the University of Rome “La Sapienza” (Ateneo Federato 2009) to L.L. The authors declare no conflicts of interest. |
Vol 161 - N° 6
P. 1004-1009 - décembre 2012 Retour au numéro
Article précédent
- 50 Years Ago in The Journal of Pediatrics : Observations on the Etiology of Acute Bronchiolitis in Infants
- Kristin Shadman
- Rate of Weight Gain and Cardiometabolic Abnormalities in Children and Adolescents
- Chadi A. Calarge, Diqiong Xie, Jess G. Fiedorowicz, Trudy L. Burns, William G. Haynes
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Déjà abonné à cette revue ?