Methicillin-resistant Staphylococcus aureus in Rio de Janeiro hospitals: Dissemination of the USA400/ST1 and USA800/ST5 SCCmec type IV and USA100/ST5 SCCmec type II lineages in a public institution and polyclonal presence in a private one - 26/02/13
Address correspondence to Kátia Regina Netto dos Santos, PhD, Laboratório de Infecções Hospitalares, Departamento de Microbiologia Médica, Instituto de Microbiologia Paulo de Góes, CCS, Bloco I, Sal 010, UFRJ, Cidade Universitária, Rio de Janeiro, RJ, Brazil.Abstract |
Background |
Methicillin-resistant Staphylococcus aureus (MRSA) infections have changed since certain non-multiresistant MRSA lineages have emerged in hospitals. In this study, 99 MRSA isolates, 77 from a public and 22 from a private hospital, were characterized.
Methods |
Isolates were tested for antimicrobial susceptibility, whereas staphylococcal chromosomal cassette mec (SCCmec) typing and Panton-Valentine leukocidin genes were assessed by polymerase chain reaction. Pulsed-field gel electrophoresis and multilocus sequence typing analyses were carried out to determine the MRSA lineages.
Results |
High rates of resistance were found to erythromycin (96%), ciprofloxacin (93%), and clindamycin (90%). The SCCmec types found were as follows: type II (14.2%), III (62.6%), and IV (23.2%). Approximately 85% of type III isolates was related to the Brazilian epidemic clone in both hospitals. For type IV isolates, 94.4% were related to both USA400/ sequence type (ST) 1 and USA800/ST5 lineages in the public hospital, whereas the USA400/ST1, USA800/ST5, USA1100/ST30, and EMRSA (Epidemic MRSA)-15/ST22 lineages were detected in the private hospital. Among the SCCmec II isolates, approximately 85% were related to the USA100/ST5 lineage. Three MRSA isolates were positive to Panton-Valentine leukocidin genes.
Conclusion |
The study showed that there was an emergence of USA400/ST1, USA800/ST5 SCCmec IV, and USA100/ST5 SCCmec II MRSA lineages in both hospitals. There was a dissemination of them in the public hospital and a polyclonal presence of the MRSA isolates in the private hospital. The spread of these lineages can be facilitated by the characteristics of the health institution.
Le texte complet de cet article est disponible en PDF.Key Words : MRSA, antimicrobial susceptibility, PFGE lineages, MLST, Public and private hospitals
Plan
| R.M.F.C. and F.S.C. contributed equally to this work. |
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| Supported by Brazilian grants from Fundação Carlos Chagas Filho de amparo à pesquisa do Estado do Rio de Janeiro (FAPERJ), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento Pessoal de Nível Superior (CAPES), Fundação Universitária José Bonifácio (FUJB), and Programa de Núcleos de Excelência (PRONEX). |
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| Conflicts of interest: None to report. |
Vol 41 - N° 3
P. e21-e26 - mars 2013 Retour au numéro
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