Oxidative Stress Negatively Affects Human Sperm Mitochondrial Respiration - 26/06/13
Abstract |
Objective |
To correlate the level of oxidative stress in serum and seminal fluid and the level of sperm deoxyribonucleic acid (DNA) fragmentation with sperm mitochondrial respiratory efficiency.
Methods |
Sperm mitochondrial respiratory activity was evaluated with a polarographic assay of oxygen consumption carried out in hypotonically treated sperm cells. A possible relationship between sperm mitochondrial respiratory efficiency, the level of oxidative stress, and the level of sperm DNA fragmentation was investigated.
Results |
Sperm motility was positively correlated with mitochondrial respiration but negatively correlated with oxidative stress and DNA fragmentation. Interestingly, sperm mitochondrial respiratory activity was negatively affected by oxidative stress and DNA fragmentation.
Conclusion |
Our data indicate that sperm mitochondrial respiration is decreased in patients with high levels of reactive oxygen species by an uncoupling between electron transport and adenosine triphosphate synthesis. This reduction in mitochondrial functionality might be 1 of the reasons responsible for the decrease in spermatozoa motility.
Le texte complet de cet article est disponible en PDF.Plan
| Financial Disclosure: The authors declare that they have no relevant financial interests. |
Vol 82 - N° 1
P. 78-83 - juillet 2013 Retour au numéro
Article précédent
- Relationship Between Testicular Sperm Extraction and Varicocelectomy in Patients With Varicocele and Nonobstructive Azoospermia
- Nicola Zampieri, Laura Bosaro, Claudio Costantini, Stefano Zaffagnini, Gianfranco Zampieri
- Comparison of Transrectal and Transvaginal Hybrid Natural Orifice Transluminal Endoscopic Surgery Partial Nephrectomy in the Porcine Model
- Wassim M. Bazzi, Sean P. Stroup, Seth A. Cohen, David M. Sisul, Michael A. Liss, James H. Masterson, Ryan P. Kopp, Suzanne R. Gudeman, Elisabeth Leeflang, Kerrin L. Palazzi, Sonia Ramamoorthy, Christopher J. Kane, Santiago Horgan, Ithaar H. Derweesh
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Déjà abonné à cette revue ?