Insulin-like growth factor 2 enhances regulatory T-cell functions and suppresses food allergy in an experimental model - 29/05/14
, Ping-Chang Yang, MD, PhD a, b, ⁎ Abstract |
Background |
The functions of regulatory T (Treg) cells are important in immunity, and the regulatory mechanisms of Treg cell activities are not fully understood yet.
Objectives |
We sought to investigate the role of insulin-like growth factor (IGF) 2 in the upregulation of Treg cell function.
Methods |
The expression of insulin-like growth factor 2 receptor (IGF2R) on T cells was assessed by using flow cytometry. Treg cell functions were evaluated by assessing the suppressor effect on proliferation of other effector T (Teff) cells. The effect of IGF2 on regulating Treg cell functions were evaluated with a cell-culture model and a food allergy mouse model.
Results |
Expression of IGF2R was observed in more than 90% of murine and human Treg cells but in less than 10% of effector CD4+ T cells. Activation of IGF2R and T-cell receptor induced marked Treg cell proliferation and release of TGF-β from Treg cells, which enhanced Treg cell immune suppressor effects on other Teff cell activities and allergic inflammation in the intestine.
Conclusions |
Activation of IGF2R enhances Treg cell functions in suppressing other Teff cell activities and inhibiting allergic inflammation in the intestine.
Le texte complet de cet article est disponible en PDF.Key words : Regulatory T cell, insulin-like growth factor, transforming growth factor, intestine, allergy
Abbreviations used : CFSE, ERK, Foxp3, IGF, IGF2R, iTreg, LPMC, MAPK, OVA, shRNA, TCR, Teff, Treg
Plan
| Supported by grants from the Natural Science Foundation of SZU (no. 000004), the Innovation of Science and Technology Commission of Shenzhen Municipality (no. JCYJ20120613172559904), the Natural Science Foundation of China (nos. 30871752, 31101280, and 81373176), the Key Laboratory Project of Shenzhen (no. SW201110010), and the Guangdong Provincial Natural Science Foundation (no. S2013040014206). |
|
| Disclosure of potential conflict of interest: The authors declare that they have no relevant conflicts of interest. |
Vol 133 - N° 6
P. 1702 - juin 2014 Retour au numéro
Article précédent
- Prostaglandin E2 resistance in granulocytes from patients with aspirin-exacerbated respiratory disease
- Tanya M. Laidlaw, Anya J. Cutler, Molly S. Kidder, Tao Liu, Juan Carlos Cardet, Heng Chhay, Chunli Feng, Joshua A. Boyce
- Accelerated dissociation of IgE-Fc?RI complexes by disruptive inhibitors actively desensitizes allergic effector cells
- Alexander Eggel, Günther Baravalle, Gabriel Hobi, Beomkyu Kim, Patrick Buschor, Patrik Forrer, Jeoung-Sook Shin, Monique Vogel, Beda M. Stadler, Clemens A. Dahinden, Theodore S. Jardetzky
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Déjà abonné à cette revue ?