Phosphatidylcholine and the intestinal mucus layer: in vitro efficacy against Clostridium difficile-associated polymorphonuclear neutrophil activation - 13/03/15
, David M. Liberati, M.S.Abstract |
Background |
Phosphatidylcholine (PC), an important component of intestinal mucus, protects against Clostridium difficile toxin-induced intestinal barrier injury in vitro. Polymorphonuclear neutrophil (PMN) activation may contribute to intestinal injury and systemic toxicity in patients with C. difficile-associated disease. We therefore hypothesized that the intestinal barrier function against C. difficile toxin by exogenous PC would ameliorate PMN activation.
Methods |
Intestinal epithelial cell (IEC) monolayers were cocultured with C. difficile toxin A and/or exogenous PC. Naïve PMNs were cocultured with IEC culture supernatants and PMN activation, and chemotactic potential determined.
Results |
PC treatment of IEC abrogated the enhanced PMN activation and chemotactic potential following toxin A exposure (P < .001).
Conclusions |
Exogenous PC ameliorated PMN activation from IECs exposed to C. difficile toxin. Administration of exogenous PC may be a useful adjunctive treatment in severely ill or immunocompromised patients with C. difficile-associated disease.
Le texte complet de cet article est disponible en PDF.Keywords : Phosphatidylcholine, PMN activation, Clostridium difficile, Chemotaxis
Plan
| Study was supported by departmental funding only. |
Vol 209 - N° 3
P. 493-497 - mars 2015 Retour au numéro
Article précédent
- Educating surgeons on intraoperative disposable supply costs during laparoscopic cholecystectomy: a regional health system's experience
- Matthew Gitelis, Yalini Vigneswaran, Michael B. Ujiki, Woody Denham, Mark Talamonti, Joseph P. Muldoon, John G. Linn
- The impact of operative timing on outcomes of appendicitis: a National Surgical Quality Improvement Project analysis
- Brett A. Fair, John C. Kubasiak, Imke Janssen, Jonathan A. Myers, Keith W. Millikan, Daniel J. Deziel, Minh B. Luu
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Déjà abonné à cette revue ?