Chicory ameliorates hyperuricemia via modulating gut microbiota and alleviating LPS/TLR4 axis in quail - 28/10/20
, Bing Zhang b, ⁎ Benvenuto su EM|consulte, il riferimento dei professionisti della salute.
L'accesso al testo integrale di questo articolo richiede un abbonamento.
| pagine | 11 |
| Iconografia | 9 |
| Video | 0 |
| Altro | 0 |
Graphical abstract |
Highlights |
• | Quail induced by high-purine diet can establish a stable model for hyperuricemia. |
• | Hyperuricemia can damage intestinal barrier and renal excretion function in quail. |
• | Chicory has strikingly hypouricemic effect and may be used as therapeutic drug. |
• | Chicory can lower uric acid by modulating gut flora and alleviating LPS/TLR4 axis. |
Abstract |
Background |
High-purine diet can cause gut microbiota disorder, which is closely related to the occurrence of hyperuricemia (HUA). At the same time, the development of HUA is often accompanied by renal impairment. Chicory, a natural medicine, has a significant effect on lowering uric acid. However, whether its concrete mechanism is associated with the regulation of gut microbiota and renal damage is still unclear.
Methods |
Hyperuricemic quails induced by high-purine diet were used, and quails were divided into control (CON), model (MOD), and model plus high, middle, low doses of chicory. The uricosuric effect was evaluated by detecting the uric acid levels in serum and feces. Meanwhile, the morphology of intestine and kidney were observed by hematoxylin and eosin (HE) staining, and the expression of intestinal barrier junction proteins Occludin, Claudin-1 were detected by quantitative real-time polymerase chain reaction (qPCR) and western blotting. Furthermore, the latent mechanism was clarified by analyzing 16S rRNA amplicon of gut microbiota and measuring the changes of LPS/TLR4 axis inflammatory response of the kidney by western blotting and enzyme-linked immunosorbent assay (ELISA).
Results |
The results showed that serum uric acid levels were significantly decreased, and the feces uric acid levels were noticeably increased after the intervention of chicory. In addition, chicory could repair the damage of intestinal mucosa and improve the permeability of intestinal barrier. Moreover, the 16S rRNA sequencing analysis uncovered that chicory restored gut microbiota by increasing the probiotics flora (Bifidobacterium, Erysipelotrichaceae) and reducing the pathogenic bacteria group (Helicobacteraceae). Furthermore, it was found that chicory reduced the LPS/TLR4 axis inflammatory response by down regulating the serum LPS and TLR4/NF-κB inflammatory pathway in kidney, thus promoting the excretion of uric acid in kidney.
Conclusion |
Chicory intervention ameliorated HUA via modulating the imbalance of gut microbiota and suppressing LPS/TLR4 axis inflammatory reaction in quail model, which may be a promising candidate for hyperuricemia-relieving properties.
Il testo completo di questo articolo è disponibile in PDF.Keywords : Hyperuricemia, Chicory, Gut microbiota, LPS/TLR4 axis, Quail
Mappa
Vol 131
Articolo 110719- novembre 2020 Ritorno al numero
Articolo precedente
- Traditional Chinese medicine protects against hypertensive kidney injury in Dahl salt-sensitive rats by targeting transforming growth factor-? signaling pathway
- Wei Liu, Yixuan Li, Xingjiang Xiong, Yuyi Chen, Lumin Qiao, Jie Wang, Xing Su, Fuyong Chu, Hongxu Liu
- Water extract of sporoderm-broken spores of Ganoderma lucidum enhanced pd-l1 antibody efficiency through downregulation and relieved complications of pd-l1 monoclonal antibody
- Jiaming He, Wenkan Zhang, Tuoyu Di, Jiahong Meng, Yiying Qi, Guoqi Li, Yuxiang Zhang, Hang Su, Weiqi Yan
Benvenuto su EM|consulte, il riferimento dei professionisti della salute.
L'accesso al testo integrale di questo articolo richiede un abbonamento.
Già abbonato a @@106933@@ rivista ?