Single versus double experimental bile duct ligation model for inducing bacterial translocation - 13/07/19
, P. Zapater-Hernández b, c, h, A. Candela-Gomis e, f, L. Compañ-Catalá g, R. Francés-Guarinos h, i, A. Payá-Romá j, A. Compañ-Rosique e, f, J. Such-Ronda g, h, k, lAbstract |
Background |
Double common bile duct ligation plus section in rats is used as a model for bacterial translocation, a phenomenon that has been correlated with the degree of liver damage. This study analyzes whether a simpler variant of the technique is also a valid model to study bacterial translocation.
Methods |
Fifty-six male Sprague Dawley rats underwent one of three surgical interventions: a) proximal double ligation and section of the common bile duct; b) proximal simple ligation of the bile duct; and c) sham operation. Bacterial translocation was measured by cultures of mesenteric lymph nodes, blood, spleen and liver. Stool culture and histological analysis of liver damage were also performed.
Results |
The incidence of bacterial translocation in SBL and DBDL groups was 23,5% and 25% respectively. Mortality was similar between ligation groups (11.2% versus 10%). Liver cirrhosis developed in the group of double ligation and section (100% of the animals at 4 weeks), while portal hypertension appeared starting at week 3. None of the animals submitted to simple ligation developed liver cirrhosis.
Conclusions |
Simple bile duct ligation is associated with a similar incidence of bacterial translocation as double ligation, but without cirrhosis or portal hypertension.
Le texte complet de cet article est disponible en PDF.Highlights |
• | SBL model does not cause cirrhosis, portal hypertension, increased inflammation or bacterial overgrowth as the DBDL model. |
• | SBL model is a good method to study BT at short term with little or no liver damage. |
• | We consider very important to describe the level at which ligatures are performed in each model used. |
Abbreviations : BT, CBD, CFU, DBDL, MLN, SBL, SBP
Plan
Vol 218 - N° 2
P. 380-387 - août 2019 Retour au numéro
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