Epidermal mammalian target of rapamycin complex 2 controls lipid synthesis and filaggrin processing in epidermal barrier formation - 05/01/20
Abstract |
Background |
Perturbation of epidermal barrier formation will profoundly compromise overall skin function, leading to a dry and scaly, ichthyosis-like skin phenotype that is the hallmark of a broad range of skin diseases, including ichthyosis, atopic dermatitis, and a multitude of clinical eczema variants. An overarching molecular mechanism that orchestrates the multitude of factors controlling epidermal barrier formation and homeostasis remains to be elucidated.
Objective |
Here we highlight a specific role of mammalian target of rapamycin complex 2 (mTORC2) signaling in epidermal barrier formation.
Methods |
Epidermal mTORC2 signaling was specifically disrupted by deleting rapamycin-insensitive companion of target of rapamycin (Rictor), encoding an essential subunit of mTORC2 in mouse epidermis (epidermis-specific homozygous Rictor deletion [RicEKO] mice). Epidermal structure and barrier function were investigated through a combination of gene expression, biochemical, morphological and functional analysis in RicEKO and control mice.
Results |
RicEKO newborns displayed an ichthyosis-like phenotype characterized by dysregulated epidermal de novo lipid synthesis, altered lipid lamellae structure, and aberrant filaggrin (FLG) processing. Despite a compensatory transcriptional epidermal repair response, the protective epidermal function was impaired in RicEKO mice, as revealed by increased transepidermal water loss, enhanced corneocyte fragility, decreased dendritic epidermal T cells, and an exaggerated percutaneous immune response. Restoration of Akt-Ser473 phosphorylation in mTORC2-deficient keratinocytes through expression of constitutive Akt rescued FLG processing.
Conclusion |
Our findings reveal a critical metabolic signaling relay of barrier formation in which epidermal mTORC2 activity controls FLG processing and de novo epidermal lipid synthesis during cornification. Our findings provide novel mechanistic insights into epidermal barrier formation and could open up new therapeutic opportunities to restore defective epidermal barrier conditions.
Le texte complet de cet article est disponible en PDF.Graphical abstract |
Key words : Epidermal barrier, mammalian target of rapamycin complex 2, Rictor, ichthyosis, filaggrin, epidermal lipid synthesis
Abbreviations used : 7-AAD, ACD, AD, APC, CE, DETC, DNFB, FACS, FFA, FITC, FLG, GFP, ICD, Krt, LB, mTOR, mTORC, myr-AKT, PE, qRT-PCR, RicEKO, Rictor, SC, SG, TCR, TEM, TEWL
Plan
| The funding source for this article was the Deutsche Forschungsgemeinschaft (Projektnummer 73111208-SFB829 and CECAD to S.A.E., S.A.W., and J.C.B. and FOR2599 to S.A.E. and A.R.) and the Center for Molecular Medicine Cologne (to S.A.E.). |
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| Disclosure of potential conflict of interest: The authors declare that they have no relevant conflicts of interest. |
Vol 145 - N° 1
P. 283 - janvier 2020 Retour au numéro
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