Shotgun metagenomic screening to improve the retrospective diagnosis of undocumented intrauterine infections on embedded placenta samples: Lessons from a brucellosis case - 15/10/23
, P.L. Woerther b, c, A. Soulier b, N. Benhaddou d, S. Seng b, M. Belan a, e, C. Rodriguez b, C. Charlier a, e, f, gHighlights |
• | Shotgun metagenomics is an emerging unbiased diagnostic approach which could be useful in case of unexplained intrauterine infection and/or unexpected fetal losses of suspected infectious origin. |
• | Shotgun metagenomics can also be used with paraffin-embedded placenta samples. |
• | Brucellosis is a rare cause of intrauterine infection. |
Abstract |
Background |
Microbiological diagnosis of intrauterine infections (IIU) still relies on bacteriological cultures or targeted DNA amplification lacking in sensitivity. Shotgun metagenomics (SMg) is an emerging unbiased molecular approach that makes it possible to sequence all the nucleic acids from any sample. It had never previously been used for IIU.
Methods |
We here report the case of a patient with an unexplained IIU and fetal loss that could be documented by a combined SMg/microbiological approach, leading to the diagnosis of maternal brucellosis.
Results |
A 31-year-old woman presented with an undocumented IIU with fetal loss at 24 weeks of gestation. Culture-based work-up failed to identify the pathogen involved. Paraffin-embedded placenta sample was retrospectively analyzed by SMg. Brucella spp nucleic acids were detected, and subacute maternal brucellosis was confirmed by targeted PCR and serological testing.
Conclusion |
This case provides grounds for further utilization of SMg for the microbiological diagnosis of unexplained obstetrical infections.
Le texte complet de cet article est disponible en PDF.Keywords : Shotgun metagenomics, Intrauterine Infection, Diagnosis, Brucella
Plan
Vol 53 - N° 7
Article 104770- octobre 2023 Retour au numéro
Article précédent
- Diagnostic accuracy of a rapid antigen triple test (SARS-CoV-2, respiratory syncytial virus, and influenza) using anterior nasal swabs versus multiplex RT-PCR in children in an emergency department
- Salim Ferrani, Thierry Prazuck, Stéphane Béchet, Fabien Lesne, Robert Cohen, Corinne Levy
- Subcutaneous antibiotic therapy use by French general practitioners: Its interest and limitations
- Emmanuel Forestier, Lea Janosch, Virginie Vitrat, Renaud Verdon, Xavier Humbert, Gaetan Gavazzi, Claire Roubaud-Baudron, on behalf of the GInGer (Groupe Infectio-Geriatrie)
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Déjà abonné à cette revue ?