Usefulness of quantitative polymerase chain reaction in amniotic fluid as early prognostic marker of fetal infection with Toxoplasma gondii - 25/08/11
, Muriel Chosson, MD b, Jacqueline Franck, MD c, Martine Wallon, MD, PhD b, François Kieffer, MD a, Karine Kaiser, PhD d, Henri Dumon, MD c, François Peyron, MD, PhD b, Philippe Thulliez, MD a, Stéphane Picot, MD, PhD d
Reprint requests: Dr Stéphane Romand, Institut de Puériculture et de Périnatalogie, Laboratoire de la Toxoplasmose, 26 boulevard Brune, 75014 Paris, France.Abstract |
Objective |
Our purpose was to evaluate Toxoplasma gondii concentration in amniotic fluid (AF) samples as a prognostic marker of congenital toxoplasmosis.
Study design |
A retrospective study was carried out in 88 consecutive AF samples from 86 pregnant women, which were found positive by prospective polymerase chain reaction (PCR) testing. Parasite AF concentrations were estimated by real-time quantitative PCR and analyzed in relation to the clinical outcome of infected fetuses during pregnancy and at birth, taking into account the gestational age at maternal infection.
Results |
A significant negative linear regression was observed between gestational age at maternal infection and T gondii DNA loads in AF. After adjusting for time at maternal seroconversion by multivariate analysis, higher parasite concentrations were significantly associated with a severe outcome of congenital infection (odds ratio [OR]=15.38/log (parasites/mL AF) [95% CI=2.45-97.7]).
Conclusion |
PCR quantification of T gondii in AF can be highly contributive for early prognosis of congenital toxoplasmosis. Maternal infections acquired before 20 weeks with a parasite load greater than 100/mL of AF have the highest risk of severe fetal outcome.
Le texte complet de cet article est disponible en PDF.Keywords : Congenital toxoplasmosis, quantitative real-time polymerase chain reaction, prenatal diagnosis, amniotic fluid
Plan
Vol 190 - N° 3
P. 797-802 - mars 2004 Retour au numéro
Article précédent
- Diet-controlled gestational diabetes mellitus does not influence the success rates for vaginal birth after cesarean delivery
- Dominic Marchiano, Mohammed Elkousy, Erika Stevens, Jeffrey Peipert, George Macones
- Elective cesarean delivery plus short-course lamivudine and zidovudine for the prevention of mother-to-child transmission of human immunodeficiency virus type 1
- Panyu Panburana, Sayomporn Sirinavin, Winit Phuapradit, Asda Vibhagool, Wasun Chantratita
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Déjà abonné à cette revue ?