Evaluation of Sperm Deoxyribonucleic Acid (DNA) Damage and Effects on Embryo Development Using a Mouse Cryptorchidism Model - 02/09/13
Abstract |
Objective |
To investigate the effects of sperm deoxyribonucleic acid (DNA) damage on fertilization and embryo development using a mouse cryptorchidism model of sperm DNA damage induction.
Materials and Methods |
Male ICR mice (aged 5-6 weeks) underwent cryptorchidism on their left testicles and sham operations on their right testicles. Spermatogenesis and sperm DNA fragmentation were assessed after 1, 2, and 4 weeks using hematoxylin-eosin staining, terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-biotin nick end labeling assays. Intracytoplasmic sperm injection into the oocytes of BDF1 females (aged 4-6 weeks) was performed using DNA-damaged sperm and normal sperm, and the fertilization rates and embryonic development were compared.
Results |
The testicular weight and size gradually decreased after induction of cryptorchidism, with progressive reduction of spermatogenesis and increased DNA damage after 1, 2, and 4 weeks. After intracytoplasmic sperm injection, the fertilization and blastocyst development rates were significantly lower in the cryptorchidism group; however, about one quarter of the embryos arising from DNA-damaged sperm continued to develop.
Conclusion |
This was an in vivo animal study to evaluate the effects of sperm DNA damage using a cryptorchidism model. Sperm DNA damage increased significantly over time after cryptorchidism. This model could be useful in investigating male factor infertility and evaluating the biologic effects of paternal DNA damage on fertilization and future embryonic development.
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| Seung-Hun Song and Jung Jin Lim contributed equally as first authors to this work. |
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| Financial Disclosure: The authors declare that they have no relevant financial interests. |
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| Funding Support: This study was supported by the Korea Healthcare Technology R&D Project, Ministry of Health, Welfare and Family affairs, Seoul Korea (grant A084923). The sponsor had no role in the design and conduct of the study, data collection, analysis, or interpretation, or preparation, review, or approval of our report. |
Vol 82 - N° 3
P. 743.e17-743.e23 - septembre 2013 Retour au numéro
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