Purification and identification of bioactive protein from leaves of Datura inoxia P.mil - 30/05/14
, Samson Kumar Shiva Shakthi a, Gajendran Babu a, Nagarathnam Radhakrishnan bAbstract |
A protein with bioactive potential was purified from the leaves of Datura inoxia P.mil. The preliminary screening of the crude proteins fractionated by Ammonium Sulphate was carried out for its antibacterial, antioxidant and anticancer properties. It exerted antibacterial activity against three bacteria namely Vibrio cholerae, Staphylococcus aureus, and Escherichia coli but was not effective against Aeromonas hydrophilia. It unveiled an increasing free radical scavenging activity in a dose-dependent manner and presented a potential anticancer activity against human colon adenocarcinoma cell line HT 29. The active protein fraction was purified by ion exchange chromatography on CM-Sephadex. The purified protein has a molecular weight of 14.3kDa and Peptide Mass Fingerprint using Matrix-Assisted Laser Desorption/Ionization-Time Of Flight Mass Spectroscopy (MALDI-TOF/MS) revealed peptide matches to defensin-like protein of Brassica napus, chaperonin CPN60-2 and mitochondria of Cucurbita maxima. The purified protein showed anticancer activity against HT 29 cancer cell line.
Le texte complet de cet article est disponible en PDF.Keywords : Antibacterial, Antioxidant, Datura inoxia, HT 29 cell line, MALDI-TOF MS
Plan
Vol 4 - N° 2
P. 143-149 - avril 2014 Retour au numéro
Article précédent
- Synthesis and in vitro anticancer evaluation of 2-isopropyl-5-methylphenol Loaded PLGA based iron oxide nanoparticles
- Balan Rajan, Shanmugam Sathish, Subramaniyan Jayakumar, Arumugam Madankumar, Krishnan Gokuladhas, Thandavamoorthy Premkumar, Ramasamy Elamaran, Mani Gopikrishnan, Thiruvengadam Devaki
- Phenolic constituents in the polar extracts of Lawsonia inermis mitigate antimycin A-induced mitochondrial degenerative cascades in Hep3B cells
- Gunjan Guha, Tamoghna Mandal, Dipita Bhakta, R. Ashok Kumar
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Déjà abonné à cette revue ?