Ghrelin upregulates PepT1 activity in the small intestine epithelium of rats with sepsis - 17/01/17
, Bin Shi b, ⁎, 1 , Kai Shi c, Guoguang Ma d, Hongze Zhang d, Xiaoli Lou e, Hongxiang Liu d, Shengxia Wan d, Dongyu Liang eBenvenuto su EM|consulte, il riferimento dei professionisti della salute.
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Highlights |
• | Reveal the effect of ghrelin on the survival rate of rats with sepsis. |
• | Confirmed the relationship between ghrelin treatment and inflammatory response in intestinal epithelial cells of rats with sepsis. |
• | Found that ghrelin treatment contributes to the physiological role of PepT1 in intestinal epithelial cells of rats with sepsis. |
Abstract |
Background |
Sepsis causes nutritional substrate malabsorption; hence, preventing gut barrier problems and improving the nutritional status in sepsis is a compelling issue.
Aims |
We tested whether ghrelin administration affects peptide transporter 1 (PepT1) activity in the intestinal epithelium of rats with sepsis.
Methods |
Sixty male Sprague-Dawley rats were randomly divided into sham-operated, sepsis, and ghrelin-treated groups. The cecum of sham-operated rats was separated after laparotomy without ligation and perforation. Sepsis group rats underwent cecal ligation and puncture (CLP). Mucosal specimens were used for immunohistochemstry, real-time PCR, and western blotting to detect PepT1 distribution, and mRNA and protein expression levels, respectively. TNF-α, IL-1β, and ghrelin levels were estimated in serum and intestinal mucosal tissue by ELISA. High-performance liquid chromatography was used to measure PepT1 uptake by the epithelial cells. Moreover, survival, body weight, and food intake of the rats were recorded during the 7-day treatment period.
Results |
All rats in the sham-operated group survived, and 80% of rats in the sepsis group died within 7d of CLP. Treatment with ghrelin attenuated the CLP-induced body weight loss, intestine mucosa damage, and the survival rate was better. In addition, ghrelin attenuated increases in TNF-α and IL-1β production. The expressions of PepT1 mRNA and protein were higher in ghrelin-treated group rats than in sepsis rats. Moreover, the uptake function of PepT1 was better in ghrelin-treated group rats.
Conclusion |
Ghrelin treatment can reduce the inflammatory response and greatly upregulate the physiological function of PepT1 in intestinal epithelial cells of rats with sepsis.
Il testo completo di questo articolo è disponibile in PDF.Keywords : Sepsis, Ghrelin, PepT1, Inflammatory response, Cecal ligation and puncture
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Vol 86
P. 669-676 - febbraio 2017 Ritorno al numero
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