Etoposide is a semi-synthetic compound derived from the plant podophyllum pelltatum and are antineoplastic agents long been used for treatment of human malignancies. The present study was conducted to examine the possible modifying effects of rosemary aqueous extract against sperm abnormalities, testes injury, DNA fragmentation, apoptosis and Ki67 alterations induced by Etoposide in male rats. A total of 50 adult male rats were divided into 5 groups (1st, control; 2nd, rosemary; 3rd, Etoposide; 4th, co-treated Etoposide with rosemary; 5th, post-treated Etoposide with rosemary). Sperms counts, motility and viability and KI67 immunoreactivity in testes were significantly decreased while; sperm abnormalities, testicular injury, P53 and DNA damage were a significantly increased in Etoposide group as compared to control group. Co-administration of rosemary with Etoposide improved the sexual toxicity, fertility potential, testicular injury, KI67, P53 and DNA damage induced by Etoposide. Etoposide treatment induced depletion in counts, motility and viability of rat sperms. Etoposide treatment induced testicular DNA damage, injury and decreased in KI67 and P53 expressions. Treatment with rosemary with Etoposide improved these alterations.