Altered fecal bacterial composition correlates with disease activity in inflammatory bowel disease and the extent of IL8 induction - 18/04/19

Doi : 10.1016/j.retram.2019.01.002

Farnaz Heidarian ^a,^b, Masoud Alebouyeh ^a,^c,^{^{1
These authors contributed equally in this study.},}^⁎ , Shabnam Shahrokh ^c,^d,^{^{1
These authors contributed equally in this study.},}^⁎ , Hedieh Balaii ^c,^d, Mohammad Reza Zali ^c,^d
^a Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran
^b Department of Biology, School of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
^c Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran
^d Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran

^⁎Corresponding author at: Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Ayatollah Taleghani Hospital, Velenjak, Shahid Aerabi Street, Yemen Street, Tehran, Iran.Foodborne and Waterborne Diseases Research CenterResearch Institute for Gastroenterology and Liver DiseasesShahid Beheshti University of Medical SciencesAyatollah Taleghani HospitalVelenjak, Shahid Aerabi StreetYemen StreetTehranIran^⁎⁎Corresponding author at: Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Ayatollah Taleghani Hospital, Shahid Beheshti University of Medical Sciences, Velenjak, Shahid Aerabi Street, Yemen Street, Tehran, Iran.Basic and Molecular Epidemiology of Gastrointestinal Disorders Research CenterResearch Institute for Gastroenterology and Liver DiseasesAyatollah Taleghani HospitalShahid Beheshti University of Medical SciencesVelenjak, Shahid Aerabi StreetYemen StreetTehranIran

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Abstract

Purpose of the study

In this study we investigated the presence and relative abundance of important genera of the gut microbiota in IBD patients and their role in induction of IL8 in a cell culture model.

Patients and methods

Stool samples of IBD patients and healthy controls were collected and relative diversity of thirteen bacterial families was measured using quantitative real-time PCR assay. Moreover, filtrate of the stool samples was used for treatment of HT-29 cell line to analyze involvement of diversity of the fecal bacterial communities in the extent of IL8 induction.

Results

Bacteroides, Faecalibacterium prausnitzii, Prevotella spp., and Methanobrevibacterium were significantly less abundant in IBD patients (UC, N = 22; CD, N = 7) compared with control group (N = 29). Increase in relative amounts of Haemophilus, Streptococcus spp., and H. pylori were detected in IBD patients, which was not statistically significant. Relative decrease in amount of Bacteroides spp., Faecalibacterium prausnitzii, and Prevotella spp. were found in UC patients with disease activity score greater than 4; however, higher levels of Streptococcus and Haemophilus were detected in the patients who were at flares. A relationship between the reduction of Haemophilus spp. and higher BMI was shown in IBD patients. Expression of IL8 was significantly higher in the treated cells by the fecal inoculates of IBD patients. Increase in relative amounts of Enterobacteriacea showed a correlation with the higher level of IL8 induction in both groups.

Conclusions

These results showed that changes in the fecal microbiota composition could affect disease activity, BMI, and IL8 induction.

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Keywords : Microbiota, Inflammatory bowel diseases, Dysbiosis, IL8HT-29 cell line

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Introduction

Materials and methods

Study design and sample collection

Relative abundance of microbiota using quantitative real-time polymerase chain reaction

PCR and real time PCR conditions for bacteria

Cell culture

Treatment of HT-29 cells with the stool samples

Quantitative real time PCR

Statistical analysis

Ethical considerations

Results

Patients and sampling

Characterization of the bacteria

Frequency of bacterial strains in fecal samples of IBD patients and healthy volunteers

Relative abundance of bacteria in IBD patients and healthy controls

Increased level of IL8 expression in the treated cell line with fecal microbiota of IBD patients compared with the controls

Discussion

Disclosure of funding

Esportazione

Vol 67 - N° 2

P. 41-50 - maggio 2019 Ritorno al numero

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Altered fecal bacterial composition correlates with disease activity in inflammatory bowel disease and the extent of IL8 induction - 18/04/19

Abstract

Purpose of the study

Patients and methods

Results

Conclusions

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