Estragole prevents gastric ulcers via cytoprotective, antioxidant and immunoregulatory mechanisms in animal models - 27/10/20
, Rodrigo de Oliveira Formiga a , Catarina Alves de Lima Serafim a , Maria Elaine Cristina Araruna a , Michele Liz de Souza Pessoa a , Roseane Carvalho Vasconcelos b , Thais Gomes de Carvalho c , Tamires Gonçalves de Jesus a , Aurigena Antunes Araújo b , Raimundo Fernandes de Araujo Junior c , Giciane Carvalho Vieira a , Marianna Vieira Sobral a , Leônia Maria Batista a, ⁎ Benvenuto su EM|consulte, il riferimento dei professionisti della salute.
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Graphical abstract |
Highlights |
• | Low oral toxicity of estragole and LD50 data was shown in this study. |
• | Estragole prevented gastric lesions in different animal models of acute induced gastric ulcers. |
• | Involvement of alternate pathways ensures the gastroprotection of estragole, such as cytoprotectant. |
• | Antioxidant and anti-inflammatory activity of estragole were also evidenced in the gastric mucosa. |
Abstract |
Background |
Estragole is an aromatic organic compound belonging to the class of phenylpropanoids derived from cinnamic aldehydes and present in essential oils of plant species, such asRavensara anisata (madeira), Ocimum basilicum (manjericão/alfavaca) and Croton zehntneri (canelinha). Pharmacological studies report its anti-inflammatory, antioxidant and vasorelaxant activity.
Hypothesis/Purpose |
This study aimed to evaluate the acute non-clinical toxicity, gastroprotective activity and the related mechanisms of action.
Methods |
Acute toxicity was assessed according to OECD guide 423 in mice. Ethanol, stress, piroxicam and pylorus ligation-induced gastric ulcer models were used to investigate antiulcer properties. The related mechanisms of action were using the ethanol-gastric lesions protocol.
Results |
In the acute oral toxicity assay, doses of 300 or 2000 mg/kg of estragole administered orally in Swiss mice did not induce any behavioral changes. However, the dose of 2000 mg/kg showed a decrease in water and feed intake. Lethal dose 50 % (LD50) was set to be equal to or greater than 2500 mg/kg, according to OECD. In all evaluated protocols, estragole (31.25, 62.5, 125 and 250 mg/kg) significantly reduced the area of ulcerative lesion when compared to control groups. To investigate the mechanisms involved in the gastroprotective activity, the antisecretory or neutralizing of gastric secretion, cytoprotectant, antioxidant and immunoregulatory effects were evaluated. Results showed that treatment with estragole (250 mg/kg) reduced (p < 0.05) the volume of the gastric juice. Besides, sulfhydryl groups, nitric oxide, mucus and prostaglandins seems to be involved in the gastroprotective property. Treatment also increased (p < 0.001) levels of reduced glutathione (GSH), interleukin-10 (IL-10) and positive cells marked for glutathione peroxidase (GPx) and cyclooxygenase 2 (COX-2). It also reduced (p < 0.001) malondialdehyde (MDA), myeloperoxidase (MPO), interleukin-1 beta (IL-1β), tumor necrosis factor-alpha (TNF-α) and inducible nitric oxide synthase (iNOS) (p < 0.05) levels.
Conclusion |
Thus, it is possible to infer that estragole presents gastroprotective activity related to antisecretory, cytoprotective, antioxidant and immunomodulatory mechanisms.
Il testo completo di questo articolo è disponibile in PDF.Abbreviations : COX-2, GPx, GSH, HE, HTAB, i.d., i.v., IL-10, IL-1β, iNOS, KATP, LD50, L-NAME, MDA, MPO, NEM, NF-kB, NO, NSAID, OECD, p.i., p.o., PAS, pH, SHs, TBO, TNF-α, ULA, ULI
Keywords : Estragole, Gastric ulcer, Gastroprotection, Cytoprotection, Antioxidant, Immunoregulatory
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Vol 130
Articolo 110578- Ottobre 2020 Ritorno al numero
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