Development of a zebrafish screening model for diabetic retinopathy induced by hyperglycemia: Reproducibility verification in animal model - 03/03/21
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Graphical abstract |
Highlights |
• | Hyperglycemia of zebrafish induction by high glucose. |
• | Zebrafish embryotoxicity and morphological change associated with hyperglycemia. |
• | High glucose increases the diameter of the zebrafish retinal vessel. |
• | Anti-inflammatory effect by aflibercept in the zebrafish diabetic retinopathy model. |
• | A new diabetic retinopathy drug can be tested in the zebrafish model. |
Abstract |
This study aimed at creating a zebrafish screening model for diabetic retinopathy, and evaluated the effects of aflibercept, which is being used to treated diabetic retinopathy. A morphological change occurred at 160 mM of glucose. The survival and hatching rate decreased in a dose-dependent manner. In the 130 mM glucose group, the retinal vessel diameter was more than double that in the normal group. The zebrafish embryo morphology changed in 200 μg/mL and 400 μg/mL at aflibercept. The survival and hatching rate decrease at 400 μg/mL. Aflibercept 100 μg/mL was a nontoxic and effective dose for the zebrafish diabetic retinopathy model. The expression of diabetic retinopathy inflammatory markers was increased in hyperglycemia. But the inflammation was improved by aflibercept in the zebrafish eye. In a zebrafish diabetic retinopathy model, the diameters of retinal vessels were reduced after treatment with aflibercept, and molecular biological and histopathological efficacy was confirmed. This model can serve for screening of new drug candidates for treatment of in diabetic retinopathy.
Il testo completo di questo articolo è disponibile in PDF.Abbreviations : DR, VEGF, IL-6, STAT3, TNF-α, IL-1 β, ZO-1, GFAP, AGE, BRB
Keywords : Diabetic retinopathy, Eye, Inflammation, Zebrafish
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Vol 135
Articolo 111201- marzo 2021 Ritorno al numero
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