Background

Delayed encephalopathy (DE) is the most severe complication after acute carbon monoxide (CO) poisoning, which seriously affects the outcome of patients and leads to a high disability rate. Prior studies have shown that hyperbaric oxygen (HBO₂) therapy is therapeutic for DE due to reducing immune-mediated neuropathology and thus improving cognitive performance.

Methods

In our present perspective study, five DE patients were treated regularly with HBO₂ therapy. The mini-mental state examination (MMSE) and Barthel index (BI) were intermittently collected during their hospitalization for mental and physical status evaluation, the peripheral bloods were serially sampled to determine the concentration changes of circulating stem cells, as well as corresponding BDNF and neural markers.

Results

MMSE and BI showed series of improvements after multiple HBO₂ therapies. The CD34⁺/CD90⁺ and CD34⁺/CD133⁺ dual positive cells, which were categorized as circulating stem cells, were observed an overall up-regulation since the beginning of the DE onset upon the application of HBO₂ therapy. Characteristic neurotrophin BDNF, neural markers such as nestin and synaptophysin (SYP) were also up-regulated after exposure of HBO₂.

Conclusion

The application of HBO₂ therapy is of significance in improving the cognition of DE patients, along with mobilized circulating stem cells. We primarily infer that the CD34⁺/CD90⁺ and CD34⁺/CD133⁺ cells were mobilized by HBO₂ exposure and have played a positive role in cognition improvement on DE patients by up-regulation of BDNF, nestin and SYP. The altering amount of circulating stem cells mobilized in peripheral blood could be a potential marker on predicting the outcome of DE.