Circular RNA circAFF2 accelerates gastric cancer development by activating miR-6894-5p and regulating ANTXR 1 expression - 14/05/21
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Highlights |
• | We found the circAFF2/miR-6894-5p/ANTXR 1/axis in gastric cancer by dual-luciferase reporter assay. |
• | We detected the expression and functions of circAFF2, miR-6894-5p, and ANTXR 1 in gastric cancer. |
• | CircAFF2 may act as an oncogene in gastric cancer and exert its effects via miR-6894-5p/ANTXR 1 signaling. |
Abstract |
Background |
Circular RNAs (circRNAs) contain a new class of non-coding RNAs that play an important role in adjusting biological function and gene expression. But the function of circRNAs in gastric cancer remains unclear. In the present research, we explored the functions of circular RNA AFF2(circAFF2, hsa_circ_0001947) in gastric cancer cells and an animal model of gastric cancer.
Methods |
The expression of circAFF2, microRNA-6894-5p (miR-6894-5p), and Anthrax toxin receptor 1 (ANTXR 1) were determined by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Cell counting kit 8 (CCK-8) and transwell assays were used to analyze the knockdown effects of circAFF2, miR-6894-5p, and overexpression of ANTXR 1 on cell proliferation, migration, and invasion abilities. Binding interactions between, circAFF2 and miR-6894-5p and between, miR-6894-5p and ANTXR 1 were detected by Dual-luciferase reporter assays. Levels of protein expression were analyzed by Western blotting. Tumor models were established by subcutaneous injection of tumor cells in nude mice.
Result |
The result showed that circAFF2 expression was significantly increased in gastric cancer cell lines and tissues. The knockdown of circAFF2 dramatically suppressed the cell migration, invasion and proliferation of gastric cancer cells. In vivo studies showed that knockdown of circAFF2 delayed tumor growth. Furthermore, we revealed that circAFF2 functioned as a sponge to absorb miR-6984-5p and elevated the expression of ANTXR 1.
Conclusion |
CircAFF2 acts as an oncogene in gastric cancer and exerts its effects via miR-6894-5p/ANTXR 1 signaling.
Il testo completo di questo articolo è disponibile in PDF.Abbreviations : CircRNAs, GC, MiRNA, CircAFF2, MiR-6894-5p, ANTXR1, SiRNA, Si-circ, Si-NC, MiR-inhi, MiR-inhi-NC, MiR-mimics, NC, PcDNA3.1
Keywords : CircAFF2, Gastric cancer, Progression, MiR-6894-5p, ANTXR 1
Mappa
Vol 45 - N° 3
Articolo 101671- maggio 2021 Ritorno al numero
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