Structural modulation of gut microbiota during alleviation of experimental passive Heymann nephritis in rats by a traditional Chinese herbal formula - 12/12/21
, Ya-Nan Yang b, 1 , Ying Liang a , Rui Lang a , Qin Zeng c , Lei Yan d , Ren-Huan Yu a, ⁎ , Chong-Ming Wu b, ⁎ Benvenuto su EM|consulte, il riferimento dei professionisti della salute.
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Abstract |
Background |
Jianpi-Qushi-Heluo formula (JQHF) has been used to treat idiopathic membranous nephropathy (IMN) in hospitals for many years.
Purpose |
Elucidating the protective effect and exploring the potential mechanism of JQHF against IMN.
Methods |
Passive Heymann nephritis (PHN) was induced in rats by a single tail vein injection of anti-Fx1A antiserum. Then, the animals were treated with JQHF at 16.2 g/kg or 32.4 g/kg, with benzepril (10 mg/kg) as a positive control. Renal function was evaluated by biochemical measurements and pathological testing. Fecal samples were collected before and after treatment to analyze the gut microbiota composition by shotgun whole metagenome sequencing.
Results |
JQHF exhibited potent efficacy in ameliorating PHN at both doses, as revealed by decreasing the deposition of IgG and C5b-9, relieving podocyte injury, and reducing glomerular and tubular cell apoptosis. The lower dose was corresponding to the clinical dosage and showed better therapeutic effects than the higher dose. Metagenomic analysis showed that gavage with 16.2 g/kg of JQHF shifted the structure of the gut microbiota in PHN rats and significantly increased the relative abundances of Prevotella copri, Lactobacillus vaginalis and Subdoligranulum variabile. Particularly, S. variabile was strongly negatively correlated with serum levels of TC and TG, the deposition of IgG and C5b-9, and apoptosis of glomerular cells.
Conclusions |
The JQHF is an effective agent for the treatment of experimental PHN. The PHN-allevating effect of JQHF is associated with specific alternation of gut microbiota.
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Highlights |
• | IMN is the leading form of idiopathic nephrotic syndrome in adults. |
• | The curative effect of JQHF is obvious for the treatment of IMN without any side effect. |
• | JQHF is effective in ameliorating of proteinuria and reduce the renal pathological damage of experimental PHN rats. |
• | Enrichment of L. vaginalis and S. variabile may partially account for the therapeutic effect of JQHF. |
Keywords : Idiopathic membranous nephropathy (IMN), Passive Heymann nephritis (PHN), Jianpi Qushi Heluo formula (JQHF), Gut microbiota, Subdoligranulum variabile
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Vol 145
Articolo 112475- gennaio 2022 Ritorno al numero
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