Chemical characterization of extracts of leaves of Kadsua coccinea (Lem.) A.C. Sm. by UHPLC-Q-Exactive Orbitrap Mass spectrometry and assessment of their antioxidant and anti-inflammatory activities - 20/04/22
Abstract |
Kadsua coccinea (K. coccinea) has long been used as a fruit and folk medicine; however, the composition of its leaves and the activities of its constituents have been seldom studied. A total of 98 chemical constituents, including 53 phenolic acids, 41 flavonoids, and 4 lignans, were identified from the plant of kadsua coccinea by UHPLC-Q-Exactive Orbitrap Mass spectrometry. All these chemicals were reported for the first time in leaves, and 95 of them have been reported for the first time in the plant of kadsua coccinea. The biological potential of extracts of K. coccinea leaves (EKL) was evaluated by in vitro antioxidant assay and anti-inflammatory assay. EKL are composed of polysaccharides (60%), polyphenols (26%), and proteins (11%). EKL present decent potent •OH and DPPH scavenging abilities and Fe2+ chelating ability. They also inhibit the secretion of NO, reduce the level of Cox2 in proteins, inhibit the secretion of pro-inflammatory cytokines, such as IL-2 and IL-6, and promote the secretion of anti-inflammatory cytokine IL-10. These results displayed significant antioxidant and anti-inflammatory activities of EKL, which will be very beneficial for further development and investigation of kadsua coccinea leaves.
Il testo completo di questo articolo è disponibile in PDF.Graphical Abstract |
Highlights |
• | A rapid and effective method based on UHPLC-Q-Exactive Orbitrap Mass spectrometry was established for the chemical identification of the leaves of K. coccinea. |
• | A total of 98 small molecules were characterized, 95 of these chemical was reported for the first time in the leaves of K. coccinea. |
• | The main components in the leaves of K. coccinea are polysaccharides and polyphenols. |
• | It displayed significant antioxidant properties and anti-inflammatory effect. |
Abbreviations : EKL;, DPPH;, •OH;, Fe2+;, PBS;, Vc;, NO;, iNOS;, Cox2;, DEX;, ELISA;, IL-2;, IL-6;, IL-10;, LPS;, DMSO;, SDS-PAGE;, PVDF;, IC50;
Keywords : Kadsua coccinea leaves, Characterization, Antioxidant activity, Anti-inflammatory activity, UHPLC-Q-Exactive Orbitrap mass spectrometry
Mappa
Vol 149
Articolo 112828- maggio 2022 Ritorno al numero
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