Cerca
image du produit
Biomedicine and pharmacotherapy

Abbonarsi

Spirulina phycocyanin extract and its active components suppress epithelial-mesenchymal transition process in endometrial cancer via targeting TGF-beta1/SMAD4 signaling pathway - 18/06/22

Doi : 10.1016/j.biopha.2022.113219 
Hsin-Yuan Chen a, b, Yi-Fen Chiang a, Chun-Yung Huang c, Tzong-Ming Shieh d, Chieh Kao e, Fu-Kuei Chang f, Tsui-Chin Huang g, Mohamed Ali h, Hsin-Yi Chang i, Yong-Han Hong b, ⁎ , Shih-Min Hsia a, j, k, l, ⁎
a School of Nutrition and Health Sciences, College of Nutrition, Taipei Medical University, Taipei 11031, Taiwan 
b Department of Nutrition, I-Shou University, Kaohsiung 84001, Taiwan 
c Department of Seafood Science, National Kaohsiung University of Science and Technology, Kaohsiung 81157, Taiwan 
d School of Dentistry, College of Dentistry, China Medical University, Taichung 40402, Taiwan 
e School of Medicine for International Students, I-Shou University, Kaohsiung 82445, Taiwan 
f Department of Health Management, College of Medicine, I-Shou University, Kaohsiung 82445, Taiwan 
g Graduate Institute of Cancer Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, Taipei 11031, Taiwan 
h Clinical Pharmacy Department, Faculty of Pharmacy, Ain Shams University, 11566 Cairo, Egypt 
i Graduate Institute of Medical Science, National Defense Medical Center, Taipei 114, Taiwan 
j School of Food and Safety, Taipei Medical University, Taipei 11031, Taiwan 
k Nutrition Research Center, Taipei Medical University Hospital, Taipei 11031, Taiwan 
l Graduate Institute of Metabolism and Obesity Sciences, College of Nutrition, Taipei Medical University, Taipei 11031, Taiwan 

⁎Corresponding author.⁎⁎Corresponding author at: School of Nutrition and Health Sciences, College of Nutrition, Taipei Medical University, Taipei 11031, Taiwan.School of Nutrition and Health Sciences, College of Nutrition, Taipei Medical UniversityTaipei11031Taiwan

Abstract

Metastasis is a major challenge in aggressive endometrial cancer treatment accounting for the high recurrence risk and poor prognosis of epithelial–mesenchymal transition (EMT), regulated by the transforming growth factor beta (TGFβ) signaling pathway, facilitates tumor metastasis. Spirulina phycocyanin extract (SPE) and its purified products allophycocyanin (APC) and C-phycocyanin (C-PC), derived from Spirulina platensis, can be considered a nutraceutical compound with the ability to inhibit tumor growth and metastasis. Current study aims to investigate the anti-metastatic potential of SPE, and its purified products APC, and C-PC on endometrial cancer both in vitro and in vivo. Firstly, human endometrial cancer cell lines (HEC-1A and Ishikawa) as an in vitro model. Secondly, HEC-1A cells transfected with luminescence gene were implanted into female nude mice as a xenograft model. MTT assay, transwell migration assay, immunoblotting assay, quantitative real-time polymerase chain reaction assay, and IVIS XRMS analysis techniques were used. The in vitro results showed that SPE and its purified products APC and C-PC inhibited cell migration, and altered the expression of EMT-related phenotypes by reversing the TGFβ/SMADs signaling pathway. The in vivo results indicated that SPE repressed the metastasis of HEC-1A-LUC cells through modulating EMT-related markers expression. Overall, SPE and its efficient components APC and C-PC reversed the EMT through targeting the TGFβ/SMADs signaling pathway, suggesting an effective therapeutic strategy for metastatic endometrial cancer.

Il testo completo di questo articolo è disponibile in PDF.

Graphical Abstract




ga1

Il testo completo di questo articolo è disponibile in PDF.

Highlights

•
This is the first study to investigate whether SPE affect the metastatic effect of invasive endometrial cancer.
•
SPE could reducing the migration of endometrial cancer cells and reducing tumor metastasis in nude mice.
•
The mechanism of SPE involve a reversal of the expression of epithelial-mesenchymal transition-related proteins and genes.

Il testo completo di questo articolo è disponibile in PDF.

Abbreviations : EC, TGFβ, EMT, SPE, APC, C-PC, R-SMADs, SNAI1, SLUG, ZEB1/2, TWIST1/2, α-SMA, TMEFF2, IVIS XRMS

Keywords : Endometrial cancer, spirulina phycocyanin extract, Allophycocyanin, C-phycocyanin, Epithelial–mesenchymal transition, Transforming growth factor beta


Mappa


© 2022  The Authors. Pubblicato da Elsevier Masson SAS. Tutti i diritti riservati.
Aggiungere alla mia biblioteca Togliere dalla mia biblioteca Stampare
Esportazione

    Citazioni Export

  • File

    •

  • Contenuto

    •

Vol 152

Articolo 113219- agosto 2022 Ritorno al numero
Articolo precedente Articolo precedente
  • FTY720 decreases ceramides levels in the brain and prevents memory impairments in a mouse model of familial Alzheimer’s disease expressing APOE4
  • Simone M. Crivelli, Qian Luo, Daan van Kruining, Caterina Giovagnoni, Marina Mané-Damas, Sandra den Hoedt, Dusan Berkes, Helga E. De Vries, Monique T. Mulder, Jochen Walter, Etienne Waelkens, Rita Derua, Johannes V. Swinnen, Jonas Dehairs, Erwin P.M. Wijnands, Erhard Bieberich, Mario Losen, Pilar Martinez-Martinez
| Articolo seguente Articolo seguente
  • Modification of taxifolin particles with an enteric coating material promotes repair of acute liver injury in mice through modulation of inflammation and autophagy signaling pathway
  • Qiteng Ding, Kecheng Chen, Xinglong Liu, Chuanbo Ding, Yingchun Zhao, Shuwen Sun, Yiwen Zhang, Jinping Zhang, Shuang Liu, Wencong Liu

Benvenuto su EM|consulte, il riferimento dei professionisti della salute.
L'accesso al testo integrale di questo articolo richiede un abbonamento.

Già abbonato a @@106933@@ rivista ?

@@150455@@ Voir plus

Il mio account

Aide & support

Dichiarazione CNIL

EM-CONSULTE.COM è registrato presso la CNIL, dichiarazione n. 1286925.

Ai sensi della legge n. 78-17 del 6 gennaio 1978 sull'informatica, sui file e sulle libertà, Lei puo' esercitare i diritti di opposizione (art.26 della legge), di accesso (art.34 a 38 Legge), e di rettifica (art.36 della legge) per i dati che La riguardano. Lei puo' cosi chiedere che siano rettificati, compeltati, chiariti, aggiornati o cancellati i suoi dati personali inesati, incompleti, equivoci, obsoleti o la cui raccolta o di uso o di conservazione sono vietati.
Le informazioni relative ai visitatori del nostro sito, compresa la loro identità, sono confidenziali.
Il responsabile del sito si impegna sull'onore a rispettare le condizioni legali di confidenzialità applicabili in Francia e a non divulgare tali informazioni a terzi.

Tutto il contenuto di questo sito: Copyright © 2026 Elsevier, i suoi licenziatari e contributori. Tutti i diritti sono riservati. Inclusi diritti per estrazione di testo e di dati, addestramento dell’intelligenza artificiale, e tecnologie simili. Per tutto il contenuto ‘open access’ sono applicati i termini della licenza Creative Commons.