Electroacupuncture activates angiogenesis by regulating the PI3K/Pten/Thbs1 signaling pathway to promote the browning of adipose tissue in HFD-induced obese mice - 13/09/23
Abstract |
This study investigated the effect of electroacupuncture (EA) on the browning of white adipose tissue (WAT) via angiogenesis and its potential mechanism in obese mice. Four-week-old male C56BL/6 mice were randomly divided into a high-fat diet (HFD) and a normal chow diet (ND) group. After 12 weeks, HFD mice were randomly divided into two groups to receive or not receive EA for 3 weeks. After EA treatment, body weight, adipocyte size, serum glucose (GLU), triacylglycerol (TG), cholesterol (CHO), leptin (Lep), monocyte chemoattractant protein-1 (MCP-1), WAT browning-related genes, angiogenesis-related genes, and the PI3K/Pten/Thbs1 signaling pathway were evaluated. The results indicated that EA significantly reduced body weight, adipocyte size, and serum concentrations of GLU, TG, CHO, Lep and MCP-1 and promoted WAT browning. Angiogenesis and the PI3K/Pten/Thbs1 signaling pathway were all activated by EA intervention. The expression levels were consistent with the results of RNA-seq and confirmed via qRT-PCR and WB. Our study showed that EA may activate angiogenesis via the PI3K/Pten/Thbs1 signaling pathway in WAT, thereby promoting the browning and thermogenesis of adipose tissue.
Il testo completo di questo articolo è disponibile in PDF.Graphical Abstract |
Highlights |
• | EA treatment significantly reduced body weight and fat accumulation. |
• | EA significantly reduced serum concentrations of obesity indicators. |
• | EA may activate angiogenesis via the PI3K/Pten/Thbs1 signaling pathway in WAT. |
• | EA promotes the browning and thermogenesis of adipose tissue. |
Keywords : Electroacupuncture (EA), White adipose tissue (WAT), Browning, Angiogenesis, Obesity
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Vol 166
Articolo 115386- ottobre 2023 Ritorno al numero
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