Tape strips detect molecular alterations and cutaneous biomarkers in skin of patients with hidradenitis suppurativa - 20/03/24
Abstract |
Background |
Hidradenitis suppurativa (HS) has a high unmet need for better treatments. Biopsies are considered the gold standard for studying molecular alterations in skin. A reproducible, minimally invasive approach is needed for longitudinal monitoring in trials and in pediatric populations.
Objective |
To determine whether skin tape strips can detect molecular alterations in HS and identify biomarkers of disease activity.
Methods |
We performed RNA sequencing on tape strips collected from lesional and healthy-appearing (nonlesional) HS skin (n = 22) and healthy controls (n = 21). We correlated the expression of skin biomarkers between tape strips and a previously published gene-signature of HS biopsies.
Results |
Tape strips detected upregulation of known HS biomarkers (eg, Interleukin[IL]-17A) in nonlesional and/or lesional skin and also identified novel clinically actionable targets, including OX40 and JAK3. The expression of Th17 and tumor necrosis factor-α pathways were highly correlated between tape strips and biopsies. HS clinical severity was significantly associated with expression of biomarkers (eg tumor necrosis factor-α , IL-17 A/F, OX40, JAK1-3, IL-4R) in HS lesional and/or nonlesional skin.
Limitations |
Sample size. Tape stripping is limited in depth.
Conclusion |
This study validates tape strips as a minimally-invasive approach to identify cutaneous biomarkers in HS. This provides a novel avenue for monitoring treatment efficacy and a potential step toward individualized therapy in HS.
Il testo completo di questo articolo è disponibile in PDF.Key words : biomarkers, hidradenitis suppurativa, IL-17, inflammation, RNA-sequencing, tape strips, TNF, transcriptome
Abbreviations used : AD, BMI, DEGs, GSVA, HS, HV, IHS4, IL, LS, NL, RNA-seq, TNF-α
Mappa
| Drs Navrazhina, Renert-Yuval and Khattri contributed equally to this work. Drs Krueger and Guttman-Yassky contribued equally as senior authors to this work. |
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| Funding sources: International Dermatology Outcome Measures (IDEOM) Hidradenitis Suppurativa Grant. |
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| Previous presentation: Preliminary data of this work has been presented as a late breaking abstract at the 2023 European Academy of Dermatology and Venereology (EADV) meeting in Berlin, Germany and the 2023 Inflammatory Skin Disease Summit (ISDS) in Vienna Austria. IDEOM meeting was in Washington, DC, USA. |
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| Patient consent: Not applicable. No recognizable patient photographs or other identifiable materials were included in this submission. |
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| IRB approval status: Reviewed and approved by institutional review board of the Rockefeller University and the Icahn School of Medicine at Mount Sinai. |
Vol 90 - N° 4
P. 749-758 - aprile 2024 Ritorno al numero
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