Mesenchymal Stem Cell Therapy Improves Diabetic Cardiac Autonomic Neuropathy and Decreases the Inducibility of Ventricular Arrhythmias - 04/12/13
, Le-Xin Wang, MD, PhD, FCSANZ b, ⁎ Riassunto |
Background |
Diabetic cardiac autonomic neuropathy (DCAN) may cause fatal ventricular arrhythmias and increase mortality in diabetics. Mesenchymal stem cells (MSCs) can secrete various cytokines and growth factors exerting neurosupportive effects. In this study, we investigated the effect of MSC on DCAN in diabetic rats.
Methods |
Forty rats were divided into normal control, diabetes mellitus (DM) control, MSC treatment (6×106 MSCs via direct myocardial injection) and MSC-conditioned medium group (100µl via direct myocardial injection). Immunohistochemistry was used to measure choline acetyltransferase (ChAT, a marker for parasympathetic nerves) and tyrosine hydroxylase (TH, a marker for sympathetic nerves) positive nerve fibres in the ventricular myocardium. Heart rate variability and programmed electrical stimulation was used to assess the inducibility of ventricular arrhythmias in the animals.
Results |
Two weeks after MSC treatment, the density of ChAT- and TH-positive nerve fibres in MSCs and MSC-conditioned medium group was higher than in DM control group (P<0.05 or P<0.01). The ChAT/TH ratio in MSC group was higher than in DM control group (0.37±0.014 vs. 0.27±0.020, P<0.01). The standard deviation of normal-to-normal R–R intervals in MSCs (5.13±0.69) and MSC-conditioned medium group (4.30±0.56) was higher than in DM control group (3.45±0.60, P<0.05). The inducibility of VAs in the MSC group was lower than in the DM control group.
Conclusions |
MSC therapy may promote cardiac nerve sprouting and increase the ratio of parasympathetic to sympathetic nerve fibres. It may also suppress the inducibility of ventricular arrhythmias in the diabetic rats.
Il testo completo di questo articolo è disponibile in PDF.Keywords : Mesenchymal stem cells, Diabetic cardiac autonomic neuropathy, Ventricular arrhythmias, Nerve sprouting, Paracrine
Mappa
Vol 22 - N° 12
P. 1018-1025 - dicembre 2013 Ritorno al numero
Articolo precedente
- Myocardial Infarction in Singapore: Ethnic Variation in Evidence-Based Therapy and Its Association with Socioeconomic Status, Social Network Size and Perceived Stress Level
- Thet Hein, Germaine Loo, Bee-Choo Tai, Qian-Hui Phua, Mark Y. Chan, Kian-Keong Poh, Boon-Lock Chia, Mark Richards, Chi-Hang Lee
- A Repeat Alcohol Septal Ablation Procedure for Hypertrophic Obstructive Cardiomyopathy Where the First Septal Branch Arose from the Intermediate (Ramus) Artery
- Grainne C. Doran, Catherine M. Burns, Dale J. Murdoch, Alexander Incani, Darren L. Walters
Benvenuto su EM|consulte, il riferimento dei professionisti della salute.
L'accesso al testo integrale di questo articolo richiede un abbonamento.
Già abbonato a @@106933@@ rivista ?